Freeze-etching: Freezing velocity and crystal size at different locations in samples
نویسندگان
چکیده
منابع مشابه
Viewed by Freeze-Etching'
The indigenous microflora of soil were released from the soil materials and concentrated without the occurrence of growth by use of a blending-simple centrifugation procedure. The cell concentrate was then frozen-etched and viewed by transmission electron microscopy. Criteria were established for detecting microbial cells among the residual soil debris. The freeze-etching of the soil cell conce...
متن کاملMembrane Splitting in Freeze-etching
The freeze-etch technique was used to observe red blood cell ghosts labeled on both surfaces with covalently bound ferritin. Ferritin molecules were never observed on fracture faces, thus indicating that fracture does not show membrane-surface detail. Subliming away the surrounding ice did expose the ferritin on the membrane surface. These results were consistent with the concept that membranes...
متن کاملRotary replication for freeze-etching
Rotary replication has been adapted to freeze-etching and evaluated using T4 polyheads, erythrocyte ghosts, and chloroplast membranes. Conventional electron microscopy, electron diffraction, and optical diffraction and filtering indicate that platinum-carbon rotary replication renders radially symmetrical contrast and 25 A resolution to freeze-etched specimens so as to clarify subunit structure...
متن کاملRecent progress in the freeze-etching technique.
The freeze-etching technique must be improved if structures at the molecular size level are to be seen. The limitations of the technique are discussed here together with the progress made in alleviating them. The vitrification of living specimens is limited by the fact that very high freezing rates are needed. The critical freezing rate can be lowered on the one hand by the introduction of anti...
متن کاملRecent progress in the freeze-etching technique
The freeze-etching technique must be improved if structures at the molecular size level are to be seen. The limitations of the technique are discussed here together with the progress made in alleviating them. The vitrification of living specimens is limited by the fact that very high freezing rates are needed. The critical freezing rate can be lowered on the one hand by the introduction of anti...
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ژورنال
عنوان ژورنال: Cryobiology
سال: 1975
ISSN: 0011-2240
DOI: 10.1016/0011-2240(75)90040-1